Technical and Scientific aspects:
Producing F1 Hybrid seeds due to a significant increase in function, price and the ability to protect natural rights reformer, most efficient and most attractive technology for vegetable seed production institutes. Modified hybrid seeds F1, depends on the commercial access of inbred lines. To develop inbred lines usually costly and time-consuming methods are used such as self-fertilization. Development of new efficient technologies such as doubled haploid can be a quick and efficient solution for producing pure lines. On the other hand access to parental inbred lines of foreign companies in the pepper producer of vegetable seeds is almost impossible and it’s a monopoly, for this reason, in current research we are following the haploid methods for reach to inbreed lines. Annual, a lot exchange of our country is used only to import hybrid vegetable seeds and the need for the production of hybrid vegetable seeds, including peppers by the Department of crop production Ministry of Agriculture has been officially announced. The economic feasibility of the project is economically perfect as well. Those items like job creation strategy, localization of new industries in agriculture and the production of hybrid seeds F1 at affordable prices and with the aim of improving the quantity and quality of pepper function, and the possibility of supply and export to the region countries and the appropriate subsequent exchange technology is also the important aspects of this project.
This study with the aim of creating a microspore embryogenesis protocol and regeneration of doubled haploid plants (genetically purified = inbred lines) is going to happen in hybrids sweet pepper by Agricultural Biotechnology Research Institute (ABRII). In order to induce embryogenesis, buds and microspores were placed in cold treatment of 4 ° C and thermal treatment of 30 and 33 ° C, for different period of time in different induction media. Based on the results, we were able to isolate adequately and appropriate microspores and induced stress and identify induction medium for our cells, create embryo cells. The embryos were transferred to regeneration medium and seedling Haploid were produced and transferred to pots and were examined cytogenetically, then their Haploid nature were determined. Grooming the haploid plants with colchicine and doubled haploid seeding which are the first seeds of inbred lines of sweet pepper in our country are produced. Now over 30 doubled haploid inbred lines produced by this way.
Now we try to produce more doubled haploid inbred lines seeds by this procedure and identify superior lines which parental inbred lines derived according to the intersection between Inbred doubled haploid line by this method and as a result, we will be successful to produce hybrid F1 seeds of sweet pepper by high quality and performance.